deltaDOT scientific team has developed considerable expertise in CE based analysis and in
solving clients’ analytical problems. Using our own HPCE LFII™ analytical instruments we offer
Analytical Services and Contract Research to industry and academia.
solving clients’ analytical problems. Using our own HPCE LFII™ analytical instruments we offer
Analytical Services and Contract Research to industry and academia.
Proteins – capillary gel electrophoresis (CGE)
Protein molecular weight calculations
Protein-protein interactions
Protein-DNA interactions
Post-translational modification analysis (PTMs)
QA/QC of vaccines ¡V impurity measurements
Excellent sensitivity (1ug/ml) for most proteins, mass accuracy of <1% and concentration
accuracy of <5%.
Proteins – Capillary zone electrophoresis (CZE)
Separation of proteins according to mass/charge ratio
Protein/Protein, Protein/Ligand interactions
Native protein analysis
PTM analysis
Antibody analysis
Analysis of both the heavy and light chains of antibodies, including glycosylation analysis on
both. Both CGE analysis (for molecular weight determination and PTM analysis) and CZE
analysis (for antibody-antigen interactions) can be carried out
Glycoform analysis
Diagnostics, therapeutics, formulation and QA/QC studies. Used in the identification of
recombinant vs. naturally occurring erythropoietin (Epo). Could be used as a test for doping in
athletes. Potential use also in monitoring drug dose of patients receiving recombinant EPO for
end stage renal failure. Transferrin glycoform analysis. Used in the identification of
carbohydrate deficient transferrin in alcoholic patients
Virus Applications
Viral Titer (deltaTITER) - kit-based baculovirus titration methodology developed jointly with
GSK. Fast and accurate titration – 18 samples can be processed in 1h and results obtained
overnight. Repeatability of titrations <10% compared with 50% obtained for traditional plaque
assays.
both. Both CGE analysis (for molecular weight determination and PTM analysis) and CZE
analysis (for antibody-antigen interactions) can be carried out
Glycoform analysis
Diagnostics, therapeutics, formulation and QA/QC studies. Used in the identification of
recombinant vs. naturally occurring erythropoietin (Epo). Could be used as a test for doping in
athletes. Potential use also in monitoring drug dose of patients receiving recombinant EPO for
end stage renal failure. Transferrin glycoform analysis. Used in the identification of
carbohydrate deficient transferrin in alcoholic patients
Virus Applications
Viral Titer (deltaTITER) - kit-based baculovirus titration methodology developed jointly with
GSK. Fast and accurate titration – 18 samples can be processed in 1h and results obtained
overnight. Repeatability of titrations <10% compared with 50% obtained for traditional plaque
assays.
Proteins
App Note
Viral Titre
App Note
LABEL FREE
INTRINSIC
IMAGING
LFII®
Enzyme analysis
Enzyme-substrate interactions.
Protein and peptide analysis
cIEF - Used to determine pI of unknown proteins (relates to the first
dimension of a 2D gel separation)
Peptide analysis - Separation and quantification of peptides in a mixture
Nucleic Acid Applications
Double stranded DNA
Analysis of PCR products and dsDNA sizing in the range of 40-1100bps
Oligonucleotide analysis
Highly sensitive oligo analysis e.g. for QA/QC (100fg/ml sensitivities
attained)
Nucleotide analysis
Analysis of deoxynucleotides in their mono, di and tri-forms
Small Molecule Applications
Chiral Molecule Analysis
Separation and analysis of enantiomeric drug molecules used in the
identification and quantification of a clinically active form of a
pharmaceutical vs. a potentially toxic form. Successfully applied to a wide
range of chiral molecules including Warfarin, Ketoprofin, Atenolol,
Propranolol.
Pesticide Analysis - compounds such as Atrazine, Carbofuran and
Parathion methyl
Water analysis - Measurement, quantification and identification of anions
and cations in water
Amino acids - CZE separation of amino acid mixtures for neo-natal
screening for inborn errors of metabolism, IVF applications.
Carbohydrates - Mono and disaccharide quantification in enzyme
kinetics
Inorganic acids - H2SO4 and similar quantification
Amino Acids
App Note
Antibody
App Note
Analytical Services
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