home technology applications about us news
deltaDOT HPCE Analytical instruments offers dramatically improved discovery power in  comparison to other conventional CE systems without the use of molecular labels. This is  achieved by the combination of substantially enhanced resolution, sensitivity and quantification  power of the instrument, along with the digital output of data delivered as clearly resolved  separations. The system has proven capability in almost all modes of CE. It allows analyte  molecular weight and concentration to be assigned and absolute measurements can be  performed using calibrating markers. Supplied with the instrument is a powerful customized  suite of data handling and analysis tools.  
deltaDOT HPCE instruments 
UV light is directed from the source via filters and optical components at the capillary window.  The detector assembly contains a photodiode array of 512 detection points.  
Optical Rail 
Capillary block 
For most electrophoretic separations a fused silica capillary coated with polyimide on the  outside is used. The separation lengths can be set from 20 cm to 70 cm by selecting the  appropriate route in the capillary block.  
Data Analysis 
The data analysis of biomolecules performed using the deltaDOT HPCE LFII® system is  enabled by proprietary Equiphase Vertexing Algorithm (EVA) and Generalised Separation  Transform (GST) algorithms. GST is a method of combining the data from the multiple pixels in  a natural way which preserves the peak shape information of the electropherograms while at  the same time maximising the signal-to-noise ratio. A factor of 10 increase in signal-to-noise  using GST as compared to using single point detection, is typically observed 
24th January 2017 – deltaDOT Ltd. announces compliance with CFR 21 part 11 of USA’s  Food and Drug Administration. deltaDOT now offers an upgraded version of its control and  analysis software which has been designed to aid users of deltaDOT’s HPCE-512 Hig  Performance Capillary Electrophoresis instrument in achieving compliance with CFR 21 part  11.The upgrade package contains controls to help meet the stringent requirements of CFR  21 part 11 including the system access by authorised personel only, maintaining audit trails  of user actions, generating complete and protected data files and the application of  electronic signatures to data (...more)  20 Sep 2016 National Health Service Quality Control North West (QCNW) Liverpool  labs  presented the data on monoclonal antibody stability trials based on deltaDOT’s Capillary  Electrophoresis (CE) technology  and expertise. They state that CE technology is suitable for  monitoring the stability of monoclonal antibodies and can perform purity and charge analysis  with high resolution and repeatability. They conclude that CE can replace other techniques  commonly used for this analysis IEC and SDS PAGE (link to the poster).  3rd July 2016 Monoclonal Antibody Stability Trials using deltaDOT HPCE-512 technology  reported by Dr. Deepika Devanur. Now available in pdf slide format on our website (...more)  4th March 2016, FDA CFR21/11 Compliance - deltaDOT’s progress continues with the  implementation of the software quality system while the P3 controller system management  software and the P3EVA analysis package had their compliance completed previously  deltaDOT engineers are working with Optra systems of California (...more)  26th January 2016, National Health Service Quality Control North West (QCNW) Liverpool  Labs are to perform monoclonal antibody stability trials, utilising deltaDOT Ltd. technology  and expertise to aid the study.     4th February 2016 Webinar: Improve your Monoclonal Antibodies Analysis. Live webinar  on analysing mAbs from production to patient. Given jointly by Horiba Scientific and  deltaDOT Ltd. Presented by Dr. Deepika Devanur (...registration site)  14th December 2015, deltaDOT technology used to define product quality for ImmBio  Ltd. deltaDOT Ltd (London) are pleased to announce that its Label Free Intrinsic Imaging  (LFII®) technology has been used to help ImmBio (Cambridge UK) to submit an  Investigational Medicinal Product Dossier (IMPD) as part of a clinical trial application to The  Medicines and Healthcare products Regulatory AgencMHRA (...more)  24th  November 2015, NW Labs are to market a kidney function test developed by  deltaDOT Ltd and the Royal Veterinary College. At the London Vet Show held at Olympia  on 19 and 20 Nov 2015 it was announced that NW Labs are now promoting deltaDOT’s  Glomerular filtration rate (GFR) assay for measuring kidney function in cats and dogs. NW  Labs will refer cases of suspected early stage renal disease for measurement by deltaDOT  with interpretation and advice on management by expert clinicians at the Royal Veterinary  College, London (...more) 12-14 March 2015, 2nd International Conference on Label-Free Technologies  Boston Marriott Cambridge, MA, USA. Dr. Stuart Hassard will be speaking on Label free  intrinsic imaging high performance capillary electrophoresis - a powerful tool in analytical  science. 4th, 5th March 2015 Keeping pace - Analytics for tomorrow’s biopharmaceuticals    conference, Leeds, UK. Dr. Stuart Hassard will be speaking on Label free intrinsic imaging  high performance capillary electrophoresis trends throughout biopharmaceutical process  analytical technology.